Opening the iTunes Store.If iTunes doesn’t open, click the iTunes application icon in your Dock or on your Windows desktop.Progress Indicator
Opening the iBooks Store.If iBooks doesn't open, click the iBooks app in your Dock.Progress Indicator

iTunes is the world's easiest way to organize and add to your digital media collection.

We are unable to find iTunes on your computer. To download from the iTunes Store, get iTunes now.

Already have iTunes? Click I Have iTunes to open it now.

I Have iTunes Free Download
iTunes for Mac + PC

When is a Heterophile Antibody Not a Heterophile Antibody? when It is an Antibody Against a Specific Immunogen (Opinion)

Clinical Chemistry 1999, May, 45, 5

This book is available for download with iBooks on your Mac or iOS device, and with iTunes on your computer. Books can be read with iBooks on your Mac or iOS device.


We enjoyed reading the interesting article entitled, "False increase in C-reactive protein attributable to heterophilic antibodies in two renal transplant patients treated with rabbit antilymphocyte globulin", by Benoist et al. (1) in this journal. The authors showed that, after treatment with rabbit antilymphocyte globulin, human anti-rabbit antibodies interfered with nephelometric/turbidimetric assays for C-reactive protein. The authors point out that this type of endogenous antibody interference with turbidimetric type assays is unusual. We do not generally think of heterophile interference as affecting turbidimetric assays. In fact, pretreatment with 40 g/L of polyethylene glycol, which reduces the IgM concentration ~80% but rarely precipitates the analyte, previously has been shown to eliminate heterophile interferences in C-reactive protein nephelometric assays (2). The article presents a question that we believe is noteworthy. Were these truly heterophile antibodies? That is to say, should specific human anti-animal (immunoglobulins) antibodies (HAAAs) be referred to as specific HAAAs rather than heterophile antibodies whenever possible? Historically, heterophile antibodies have been sheep cell agglutinins associated with mononucleosis. These antibodies are developed against poorly defined immunogens. "Hetero" and "phile" are from the Greek, and mean "different" and "affinity", respectively. The Taber's Medical Dictionary defines them as an antibody response to an "antigen other than the specific one" (3). In 1973, a classic paper identifying endogenous antibody interference was published by Prince et al. (4), who demonstrated the occurrence of false-positive results in the two-site immunometric assay for hepatitis B antigen. The interference was produced by the bridging of guinea pig antibodies used as capture and detection antibodies in the assay by a human antibody. Of note is that these investigators showed that the interference could be simulated to varying degrees by antibodies from several species and could be reduced by treating the sample with nonimmune guinea pig globulin.

When is a Heterophile Antibody Not a Heterophile Antibody? when It is an Antibody Against a Specific Immunogen (Opinion)
View in iTunes
  • $5.99
  • Available on iPhone, iPad, iPod touch, and Mac.
  • Category: Chemistry
  • Published: 01 May 1999
  • Publisher: American Association for Clinical Chemistry, Inc.
  • Seller: The Gale Group, Inc.
  • Print Length: 9 Pages
  • Language: English
  • Requirements: To view this book, you must have an iOS device with iBooks 1.3.1 or later and iOS 4.3.3 or later, or a Mac with iBooks 1.0 or later and OS X 10.9 or later.

Customer Ratings

We have not received enough ratings to display an average for this book.