Suppressive Effects of Swainsonine on C6 Glioma Cell in Vitro and in Vivo (Short Communication) (Report)
Phytomedicine: International Journal of Phytotherapy & Phytopharmacology 2009, Nov, 16, 11
Phytomedicine: International Journal of Phytotherapy & Phytopharmacology
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Abstract Swainsonine, an extract from Astragalus membranaceus, is known for its anti-cancer -effects and could prevent metastases. In order to investigate the effects and mechanisms of swainsonine in C6 glioma cells, we carry out correlated experiments in vitro and in vivo. After treatment with swainsonine, the effective dose and [IC.sub.50] value of swainsonine in the C6 glioma cell were examined using the MTT assay. Cell cycle distribution and apoptotic rates were analyzed using FCM and [Ca.sup.2+][sub.i] was measured by LSCM. Expressions of pl6 and p53 protein were evaluated by immunocytochcmical methods. Simultaneously, glioma-bearing rats were administered swainsonine at doses of 2, 4 and 8 mg/kg body wt. The inhibition rate was calculated and pathological sections were observed. The results indicated that the growth of C6 glioma cells is inhibited by swainsonine in vitro, with an [IC.sup.50] value within 24 h of 0.05 [micro]g/ml. Increases in swainsonine correlate with S phase percentages of 11.3%, 11.6% and 12.4%, respectively. Moreover, the expression of apoptosis inhibiting p53 and pi 6 protein decreases gradually. Tumor weight in vivo decreased clearly and HE dyeing of tumor tissue showed gray, its texture was soft, with necrosis and hemorrhagic concentrated inward. Swainsonine could inhibit the proliferation of C6 glioma cells in vitro and the growth of C6 glioma in vivo. The mechanisms of swainsonine-induced apoptosis may relate with the expression of apoptosis-related genes and overloading-[[Ca.sup.2+].sub.i]-induced endoplasmic reticulum stress.
- 2,99 €
- Category: Life Sciences
- Published: 01 November 2009
- Publisher: Urban & Fischer Verlag
- Print Length: 15 Pages
- Language: English